Components

2g pT7Blue^™ T-Vector, 50 ng/l 25 ng T-Vector Positive Control Insert, 2.5 ng/l

General description

The pT7Blue^™ T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3′ A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue^™ vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3′ dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3′ dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA﹤TMSYMBOL>﹤/TMSYMBOL> Tailing Kit. pT7Blue^™ contains the pUC19 backbone (including high-copy number origin or replication and lac sequences), a T7 promoter, f1 origin of replication, and a modified multiple cloning region.

Legal Information

This product is only available in Japan.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

PT7BLUE is a trademark of EMD Chemicals Inc.

Packaging

1 kit in Plastic ampoule

Warning

Toxicity: Standard Handling (A)